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SBAQ Molecular Biology of the Cell

1. A 65-year-old man with a history of chronic alcoholism is admitted with confusion and ataxia. Thiamine deficiency is suspected. Which cofactor-dependent step in carbohydrate metabolism would be most directly impaired?

AFructose-6-phosphate to fructose-1,6-bisphosphate
PFK-1 - the rate-limiting step of glycolysis. Driven by ATP, not a thiamine cofactor.
B1,3-bisphosphoglycerate to 3-phosphoglycerate
Phosphoglycerate kinase (substrate-level ATP synthesis). No thiamine involved.
CPyruvate to acetyl CoA by pyruvate dehydrogenase
PDH uses thiamine pyrophosphate (TPP) as its cofactor - the link between glycolysis and the TCA cycle. Blocked in deficiency, the basis of Wernicke's encephalopathy.
DGlucose-6-phosphate to fructose-6-phosphate
Phosphoglucose isomerase - a simple isomerisation with no cofactor of this kind.
EGlyceraldehyde-3-phosphate to 1,3-bisphosphoglycerate
GAPDH - depends on NAD⁺ and inorganic phosphate, not thiamine.
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